Accurate particle analysis in heterogeneous biological fluids such as serum, plasma, urine and cell lysates remains challenging for conventional light scattering methods including DLS, SLS, NTA and Light Obscuration.

SPES technology enables true single-particle characterization directly in complex biological media, supporting drug delivery studies, nanoparticle analysis and eco-/cytotoxicity investigations.

Example of application: Study of drug carriers based on PLGA particles in human plasma

PLGA particles are widely used as drug delivery carriers.

SPES technology characterizes PLGA particles before and after incubation in human plasma, monitoring changes in optical properties, ageing processes and PEG stabilization, providing valuable information for formulation development.

 

(In figure) (Top)

EOS CLOUDS maps demonstrate optical fingerprint changes of PLGA particles after incubation in human plasma, highlighting ageing effects and PEG stabilization.

 

Example of application: Identification of NanoPlastic particles in unfiltered cell lysate

SPES technology enables direct identification of nanoplastic particles in complex biological matrices including cell lysates and culture supernatants, allowing investigation of particle uptake and biological interactions.

 

(In figure) EOS CLOUDS distinguish particle populations in supernatants and cell lysates, revealing biological interactions through changes in optical properties.

 

Discover more > EOS Application Note AN007 Quantitative Classification of Particles in BioFluids via SPES Technology