Particle characterization in biological complex media (serum, blood, urine, as well as cell culture medium) is foreclosed to traditional technologies because of the presence with relatively high concentration of a heterogeneous, polydisperse submicron component, as proteins, lipids, antibodies, vitamins which commonly share with particles the same size, especially if aggregates are present.

CLASSIZER™ ONE is the unique available instrument on the market able to distinguish between signals as coming from the test particles or other different components of the complex mixture. In this way, it is possible to easily perform analysis directly in the complex fluid where the particles are dispersed, without purification or filtration steps, which tipically are time-consuming techniques and alter the systems.

The case of polymeric particles in mouse serum and blood

Standard polystyrene (PS) particles have been easily detected and analyzed by CLASSIZER ONE™ even when suspended in media as complex as mouse serum. Differently from traditional technologies, the strong background given by the serum does not prevent CLASSIZER™ ONE from measuring the PS spheres with high resolution, even at low concentration.
Noteworthy, a fine size distribution analysis can be obtained in the same experimental conditions (concentration as low as 10E5 particles/mL in mouse serum) also for a polydisperse sample of biodegradable poly(lactic-co-glycolic acid) (PLGA). CLASSIZER™ ONE opens up new opportunities in particle-kinetics, dosimetry, life science, and more.

ApplicationComplexMedia00_new

On the contrary, SPES technology distinguishes between signals as coming from different components of a complex mixture. In this way, it is possible to easily perform analysis directly in the complex fluid where the particles are dispersed. A great improvement in the particle engineering process is possible:

Analysis

Analysis can be done at every steps of the particle production, even without purification

Easily and in-line

Particles are easily and in-line discriminated from the biological components

Immediately

Particle aggregation is immediately detected

Monitored

The biomolecular corona formation, when particle size increase adequately, is monitored